Application of organoid technologies in colorectal cancer modeling and developing precision medicine
沈家寧
Chia-Ning Shen
沈家寧 Chia-Ning Shen
Research Fellow Professor
Current Position
Genomics Research Center, Academia Sinica, Taipei
Education
Curriculum Vitae
Dr. Chia-Ning Shen is currently a research fellow in the Genomics Research Center, Academia Sinica, Taiwan. Dr. Shen earned his PhD from Developmental Biology Program, Department of Biology and Biochemistry, University of Bath, United Kingdom in 2002. From 2002 to 2004, Dr. Shen worked as a research officer in the Centre for Regenerative Medicine in University of Bath. Dr. Shen came back to Taiwan and joined the Genomics Research Center of Academia Sinica in the summer of 2004. Dr. Shen's research interest primarily focuses on two fields, cancer stem cells and regenerative medicine. He currently focuses on investigating mechanisms involved in naturally occurring somatic cell reprogramming. Based dissecting the basis of cell differentiation and transdifferentiation, Dr. Shen aims to develop methodologies to trigger tissue regeneration and to identify the initial factors for neoplastic transformation in somatic cells. For example, Dr Shen is applying organoid technologies to dissect cancer causes and to develop precision medicinal strategies. As of today, Dr. Shen has published over 90 articles in world-renowned journals which have been cited for more than 3600 times.
Dr. Shen had spared time to attend several training courses. In 2008, he has completed the course program in legal studies in National Taiwan University. In 2012, he also attended the international training program of Multidisciplinary Management of Technology in National Cheng-Chi University. Aside from conducting research, Dr. Shen has also been responsible for coordinating academic activities and overseeing administrative work at the Genomics Research Center as the Deputy Director from 2013 to 2019. He has also served as Acting Division Director of Biotechnology Incubation Center in the Genomics Research Center for incubating biotechnology companies that licensed technologies from Academia Sinica. Dr. Shen joined Biomedical Translation Research Center in September 201. From 2019-2022, Dr. Shen served as CEO of BioHub Taiwan (Innovation & Incubation Center, Biomedical Translation Research Center) to establish Biotech Ecosystem and to incubate biotech startups at National Biotechnology Research Park. Currently, Dr. Shen is a chief PI managing the core facilities for translational medicine at National Biotechnology Research Park.
Dr. Shen was involved in the establishment of Taiwan Society for stem cell research (TSSCR) in 2005 aiming at providing opportunities for close interactions among stem cell researchers in Taiwan. Dr. Shen has been served as TSSCR president from 2017 to 2021 to cooperates with the government to implement relevant cell therapy legislation and policies. Dr. Shen is currently an Executive Supervisor in TSSCR.
陳天華
Tien-Hua, Chen
10:45 - 11:00
致德堂
大會開幕式
11:00 - 12:00
大會特別演講致德堂
Glucose metabolism and its signaling in cancer and immune regulation
林慧觀
Hui-Kuan Lin
林慧觀 Hui-Kuan Lin
Director of Prostate Cancer Center
Current Position
Department of Cancer Biology
Wake Forest University School of Medicine
Education
National Taiwan University, Taipei, Taiwan, Taiwan, BS, 1993, Pharmacy
National Taiwan University, Taipei, Taiwan, Taiwan, MS, 1995, Pharmacology
University of Rochester, Rochester, NY, PHD, 2002, Pathology (Cancer Biology)
Curriculum Vitae
Zhang, W., Wang, G., Xu, ZG., Tu, F., Dai, J., Chang, Y., Chen, Y., Lu, Y., Zeng, H., Cai, Z., Han, F., Xu, C., Jin, G., Sun, L., Pan, BS., Lai, SW., Hsu, CC., Xu, J., Chen, Z., Li, HY., Seth P., Hu, J., Zhang, X., Li, H., Lin, HK. Lactate is a natural suppressor of RLR signaling by targeting MAVS. Cell. 2019 Jun 27;178(1):176-189.e15. doi: 10.1016/j.cell.2019.05.003. Epub 2019 May 30.
Wang G, Long J, Gao Y, Zhang. W, HanF, XuC, SunL, YangSC, Lan J, HouZ, CaiZ, Jin G, Hsu CC, Wang YH, Hu J, Chen TY, Li H, Lee MG, Lin HK.SETDB1-mediated methylation of Akt promotes its K63-linked ubiquitination and activation leading to tumorigenesis. Nat. Cell Biol. 2019 Feb;21(2):214-225.
Chan CH, Morrow JK, Li CF, Gao Y, Jin G, Moten A, Stagg LJ, Ladbury JE, Cai Z, Xu D, Logothetis CJ, Hung MC, Zhang S, Lin HK. Pharmacological Inactivation of Skp2 SCF Ubiquitin Ligase Restricts Cancer Stem Cell Traits and Cancer Progression. Cell 154(3):556-68, 8/2013.
Chan CH, Li CF, Yang WL, Gao Y, Lee SW, Feng Z, Huang HY, Tsai KK, Flores LG, Shao Y, Hazle JD, Yu D, Wei W, Sarbassov D, Hung MC, Nakayama KI, Lin HK. The Skp2-SCF E3 Ligase Regulates Akt Ubiquitination, Glycolysis, Herceptin Sensitivity, and Tumorigenesis. Cell 149(5):1098-111, 5/2012.
Yang WL, Wang J, Chan CH, Lee SW, Campos AD, Lamothe B, Hur L, Grabiner BC, Lin X, Darnay BG, Lin HK. The E3 ligase TRAF6 regulates Akt ubiquitination and activation. Science 325(5944):1134-1138, 8/2009.
林琬琬
Wan-Wan Lin
12:00 - 14:00
科技新知研討會
午餐 科技新知研討會-諾倫科技
黃元孝
HUANG,YUAN-XIAO
黃元孝 HUANG,YUAN-XIAO
Current Position
Education
Curriculum Vitae
諾倫科技股份有限公司 執行長 黃元孝
BS & MS in Chemical & Biomolecular Engineering, Johns Hopkins University
專業領域：研究病理學/癌症藥物開發/藥物篩檢與醫學工具開發
特殊經歷：擔任美國Broad Institute/Calico/AbbVie三方合作癌症惡質症藥物
研發工程師
許筑瑩
SHIU,JU-YING
12:00 - 14:00
壁報論文展示
13:00 - 14:20
陳烱霖轉譯醫學講座特別演講致德堂
Communication between cancer cells and immune cells during tumor progression
楊慕華
Muh-Hwa Yang
楊慕華 Muh-Hwa Yang
Chair professor of Institute of Clinical Medicine, Yang Ming Chiao Tung University
Current Position
Institute of Clinical Medicine, National Yang Ming Chiao Tung University
Education
M.D., National Yang-Ming University
Ph.D., National Yang-Ming University
Resident and clinical fellow of Division of Hematology-Oncology, Taipei Veterans General Hospital
Curriculum Vitae
Chen HY, Hsieh, CH, Lin PH, Chen YT, Hsu DS, Tai SK, Chu PY, Yang MH*. Snail regulated microRNA-21 suppresses NLRP3 inflammasome activity to enhance cisplatin resistance. J Immunother Cancer 2022;10:e004832.
Ou DL, Chen CW, Hsu CL, Chung CH, Feng ZR, Lee BS, Cheng AL, Yang MH*, Hsu C*. Regorafenib enhances antitumor immunity via inhibition of p38 kinase/Creb1/Klf4 axis in tumor-associated macrophages. J Immunother Cancer 2021;9:e001657.
Liao TT, Lin CC, Jiang JK, Yang SH, Teng HW, Yang MH*. Harnessing stemness and PD-L1 expression by AT-rich interaction domain-containing protein 3 in colorectal cancer. Theranostics 2020;10:6095-6112.
Li CF, Chen JY, Ho YH, Hsu WH, Wu, LC, Lan HY, Hsu DS, Tai SK, Chang YC*, Yang MH*. Snail-induced claudin-11 prompts collective migration for tumor progression. Nat Cell Biol 2019;21:251-262.
Hwang WL*, Lan HY, Cheng WY, Huang SC, Yang MH*. Tumor stem-Like cells-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer. J Hematol Oncol 2019;12:10.
鄭子豪
TH Cheng
14:30 - 15:30
會員大會
14:30 - 16:40
致德堂
大會主題論文競賽
15:30 - 15:40
Café Break
15:40 - 16:20
多元應用創新研究
The Combination of Stem Cells and Three-dimensional Printed PGSA Scaffolds for Vascular Tissue Engineering Applications
蔣偉程
Wei-Cheng Jiang
蔣偉程 Wei-Cheng Jiang
Assistant Professor, Department of Anatomy and Cell Biology, School of Medicine, National Yang Ming Chiao Tung University 國立陽明交通大學醫學系解剖學及細胞生物學科 助理教授
Current Position
Department of Anatomy and Cell Biology, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan
Education
Curriculum Vitae
Chen CH, Ho HH, Jiang WC, Ao-Ieong WS, Wang J, Orekhov AN, Sobenin IA, Layne MD, Yet SF.Cysteine-rich protein 2 deficiency attenuates angiotensin II-induced abdominal aortic aneurysm formation in mice. J Biomed Sci. 2022;12;29(1):25.
Jiang WC, Hsu WY, Ao-Ieong WS, Wang CY, Wang J, Yet SF. A novel engineered vascular construct of stem cell-laden 3D-printed PGSA scaffold enhances tissue revascularization. Biofabrication. 2021;13(4):045004.
Jiang WC†, Chen CM†, Hamdin CD, Orekhov AN, Sobenin IA, Layne MD, Yet SF. Therapeutic Potential of heme oxygenase-1 in aneurysmal diseases.Antioxidants (Basel). 2020;9(11):1150. [†, equal contribution]
Lai YL†, Lin CY†, Jiang WC†, Ho YC, Chen CH, Yet SF. Loss of heme oxygenase-1 accelerates mesodermal gene expressions during embryoid body development from mouse embryonic stem cells. Redox Biol. 2018;15:51-61. [†, equal contribution]
Jiang WC, Cheng YH, Yen MH, Chang Y, Yang VW, Lee OK. Cryo-chemical decellularization of the whole liver for mesenchymal stem cells-based functional hepatic tissue engineering. Biomaterials. 2014;35(11):3607-3617.
許鍾瑜
Jung-Yu Hsu
16:20 - 17:00
多元應用創新研究
The Effect of RET Activation in mice Retina
彭偉豪
Peng Wei Hao
彭偉豪 Peng Wei Hao
Assistant Professor in School of Medicine and Institute of Biotechnology, College of life science and medicine, National Tsing Hua University 清華大學 生命科學暨醫學院 學士後醫學系/生物科技研究所 助理教授
Current Position
School of Medicine and Institute of Biotechnology, College of life science and medicine, National Tsing Hua University
Education
Ph.D., Graduate Institute of Anatomy & Cell Biology, National Taiwan University.
B.S., Department of Physical Therapy, Chung Shan Medical University
台灣大學醫學院解剖學暨細胞生物學研究所博士
中山醫學大學物理治療學系學士
Curriculum Vitae
1. Peng WH, Kan HW, Ho YC: "Periaqueductal gray is required for controlling chronic stress-induced depression-like behavior", Biochem Biophys Res Commun, vol. 593, pp. 28-34, 2022.02
2. Liao ML, Kung HN, Lu KS, Shen JH, Peng WH: Alterations in the von Ebner's gland secretion and implications for taste sensation in diabetic (db/db) mice. Histology and histopathology 2022, 37(1):69-79 .
3. Peng WH, Liao ML, Huang WC, Liu PK, Levi SR, Tseng YJ, Lee CY, Yeh LK, Chen KJ, Chien CL, Wang NK: Conditional Deletion of Activating Rearranged During Transfection Receptor Tyrosine Kinase Leads to Impairment of Photoreceptor Ribbon Synapses and Disrupted Visual Function in Mice. Frontiers in Neuroscience 2021, 15:728905
4. Lee NC, Peng WH, Tsai LK, Lu YH, Wang HC, Shih YC, Pung ZX, Hu HY, Hwu WL, Tseng WI, Chien YH: Ultrastructural and diffusion tensor imaging studies reveal axon abnormalities in Pompe disease mice. Scientific Reports 2020, 10(1):202395.
5. Liao ML, Peng WH, Kan D, Chien CL: Distribution patterns of the zebrafish neuronal intermediate filaments inaa and inab. Journal of Neuroscience Research 2019, 97(2):202-214.
許鍾瑜
Jung-Yu Hsu
17:00 - 17:40
多元應用創新研究
Operation nuclear storm: Blocking the “TYRO3” missile to ameliorate colon cancer malignancy
許佩玲
Pei-Ling Hsu
許佩玲 Pei-Ling Hsu
Current Position
Department of Anatomy, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan
Sheng-Feng Tsai, Pei-Ling Hsu,Yun-Wen Chen, Mohammad Shahadat Hossain, Pei-Chun Chen, Shun-Fen Tzeng, Po-See Chen, Yu-Min Kuo*. High-fat diet induces depression-like phenotype via astrocyte-mediated hyperactivation of ventral hippocampal glutamatergic afferents to the nucleus accumbens. Molecular Psychiatry.2022. 27(11):4372-4384.
Wan-Ning Li, Kuei-Yang Hsiao, Chu-An Wang, Ning Chang, Pei-Ling Hsu, Chung-Hsien Sun, Shang-Rung Wu, Meng-Hsing Wu, Shaw-Jenq Tsai*. Extracellular vesicle-associated VEGF-C promotes lymphangiogenesis and immune cells infiltration in endometriosis. Proceedings of the National Academy of Sciences of the United States of America. 2020. 117(41):25859-25868.
Pai-Sheng Chen, Wen-Tai Chiu, Pei-Ling Hsu, Shih-Chieh Lin, I-Chen Peng, Chia-Yih Wang, Shaw-Jenq Tsai*. Pathophysiological implications of hypoxia in human diseases. Journal of Biomedical Science. 2020. 27(1):63.
Chu-An Wang, I-Heng Chang, Pei-Chi Hou, Yu-Jing Tai, Wan-Ning Li, Pei-Ling Hsu, Shang-Rung Wu, Wen-Tai Chiu, Chien-Feng Li, Yan-Shen Shan, Shaw-Jenq Tsai*. DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination. Journal of Extracellular Vesicles. 2020. (1):1746529.
Anterior gradient 2 induces resistance to sorafenib via endoplasmic reticulum stress regulation in hepatocellular carcinoma
陳政義
Cheng-Yi Chen
陳政義 Cheng-Yi Chen
Assistant professor 助理教授
Current Position
Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University
Education
2004. 9 - 2006. 6 M.S., Anatomy and Cell Biology, National Yang-Ming University, Taipei, Taiwan
2006. 9 - 2012. 6 Ph.D., Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan
2013. 8 - 2020. 1 Assistant Research Fellow, Department of Medical Research, Mackay Memorial Hospital, Taipei, Taiwan
2020. 2 ~ present Assistant professor, Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan
Curriculum Vitae
Hepatocellular carcinoma (HCC) accounts for almost 80% of all liver cancers and is the sixth most common cancer and the second most common cause of cancer-related death worldwide. The survival rate of sorafenib-treated advanced HCC patients is still unsatisfactory. Unfortunately, no useful biomarkers have been verified to predict sorafenib efficacy in HCC. We assessed a sorafenib resistance-related microarray dataset and found that anterior gradient 2 (AGR2) is highly associated with overall and recurrence-free survival and several clinical parameters in HCC. However, the mechanisms underlying the role of AGR2 in sorafenib resistance and HCC progression remain unknown. We found that sorafenib induces AGR2 secretion via posttranslational modification, and AGR2 plays a critical role in sorafenib-regulated cell viability and endoplasmic reticulum (ER) stress and induces apoptosis in sorafenib-sensitive cells. Sorafenib downregulated intracellular AGR2 and conversely induces AGR2 secretion, which suppresses its regulation of ER stress and cell survival in sorafenib-sensitive cells. In addition, AGR2 is highly intracellularly expressed in sorafenib-resistant cells, which supports ER homeostasis and cell survival. We suggest that AGR2 regulates ER stress to influence HCC progression and sorafenib resistance. This is the first report that AGR2 can modulate ER homeostasis via the IRE1α-XBP1 cascade to regulate HCC progression and sorafenib resistance. Elucidation of the predictive value of AGR2 and its molecular and cellular mechanisms in sorafenib resistance could provide additional opportunities for HCC treatment.
馮琮涵
Tsorng-Harn Fong
14:10 - 14:50
New Strategies for Cancer Therapy
Functional roles of mucin-type O-glycosylation in gastric cancer
陳學亭
Syue-Ting Chen
陳學亭 Syue-Ting Chen
Assistant Professor, Department of Anatomy, College of Medicine, Chang Gung University 長庚大學醫學院解剖學科-助理教授
Current Position
Department of Anatomy, College of Medicine, Chang Gung University
Education
Ph.D./ Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University
M.S./ Graduate Institute of Anatomy and Cell Biology, College of Medicine, National Taiwan University
Curriculum Vitae
1. Chang KS#, Chen ST#, Sung HC, Hsu SY, Lin WY, Hou CP, Lin YH, Feng TH, Tsui KH*, and Juang HH* (2022, Sep).
WNT1 Inducible Signaling Pathway Protein 1 Is a Stroma-Specific Secreting Protein Inducing a Fibroblast Contraction and Carcinoma Cell Growth in the Human Prostate. International Journal of Molecular Sciences, 23, 11437.
2. Sung HC#, Chang KS#, Chen ST, Hsu SY, Lin YH, Hou CP, Feng TH, Tsui KH*, and Juang HH* (2022, Aug). Metallothionein 2A with Antioxidant and Antitumor Activity Is Upregulated by Caffeic Acid Phenethyl Ester in Human Bladder Carcinoma Cells. Antioxidants, 11(8), 1509.
3. Hou CP#, Tsui KH#, Chen ST, Chang KS, Sung HC, Hsu SY, Lin YH, Feng TH and Juang HH* (2022, Jul). The Upregulation of Caffeic Acid Phenethyl Ester on Growth Differentiation Factor 15 Inhibits Transforming Growth Factor β/Smad Signaling in Bladder Carcinoma Cells. Biomedicines, 10(7), 1625.
4. Lee PC, Chen ST, Kuo TC, Lin TC, Lin MC, Huang J, Hung JS, Hsu CL, Juan HF, Lee PH, Huang MC*. C1GALT1 is associated with poor survival and promotes soluble Ephrin A1-mediated cell migration through activation of EPHA2 in gastric cancer. Oncogene. 2020 Mar;39(13):2724-2740.
5. Chen ST, Kuo TC, Liao YY, Lin MC, Tien YW*, Huang MC*. Silencing of MUC20 suppresses the malignant character of pancreatic ductal adenocarcinoma cells through inhibition of the HGF/MET athway. Oncogene. 2018 Nov;37(46):6041-6053.
馮琮涵
Tsorng-Harn Fong
14:50 - 15:30
New Strategies for Cancer Therapy
The role of hedgehog signaling in paclitaxel sensitivity of EGFR wild-type non-small cell lung cancer
蘇柏全
Bor-Chyuan Su
蘇柏全 Bor-Chyuan Su
Department of Anatomy and Cell Biology, School of Medicine, Taipei Medical University Assistant Professor 臺北醫學大學醫學系解剖學暨細胞生物學科 助理教授
Current Position
Department of Anatomy and Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan.
Education
國立成功大學基礎醫學研究所 博士
Curriculum Vitae
Non-small cell lung cancer (NSCLC) is a common and deadly disease around the world. For the nearly 50% of NSCLC patients with tumors harboring wild-type epidermal growth factor receptor (EGFR WT), therapeutic options are highly limited, and chemotherapy is the primary treatment. Compounding this issue, EGFR WT NSCLC patients usually have poor response to chemotherapy. Thus, new therapeutic strategies are urgently needed to improve chemotherapy response in EGFR WT NSCLC patients. Previous work demonstrated that hedgehog signaling is highly induced in NSCLC, and its expression levels is associated with poor clinical outcome. However, its role in chemotherapy response of EGFR WT NSCLC patients is still unclear. In the present study, we demonstrate that hedgehog signaling is induced by paclitaxel (PTX) in EGFR WT NSCLC cells. Furthermore, suppression of hedgehog signaling by a specific hedgehog inhibitor, GDC-0449 (Vismodegib), increases NSCLC cell sensitivity to PTX. GDC-0449 also enhances PTX-induced mitochondrial damage and reactive oxygen species production. We also found that hedgehog induces Akt phosphorylation, which in turn phosphorylates Bax at Ser184. This phosphorylation event switches the activity of Bax from pro- to anti-apoptotic, promoting resistance of EGFR WT NSCLC cells toward PTX. Together, our findings suggest that inhibition of hedgehog might be a promising strategy to enhance the therapeutic sensitivity of EGFR WT NSCLC to PTX.
